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Virologica Sinica, 07 (3) : 357, 1992
Review
Restriction Enzyme Analysis and Molecular Cloning of CAV-1 DNA
The National Veterinary biotechnological Laboratory
Harbin Vet. Res. Inst. Harbin 150001
The Military Veterinary Research Institute
Vet. College of PLA
Changchun 130012
 Correspondence:
(132.41KB)  
Abstract
The virulent and modified canine adenovirus type 1 (CAV-1) DNAs extracted from canine kidney cells (MDCK) 36 hrs postinfection were both analysed with restriction enzymes EcoR Ⅰ, BamH Ⅰ, Pst Ⅰ, Nsi Ⅰ, Sph Ⅰ and Hind Ⅲ. and no difference was found from the electrophoresis pattern between the two viruses. The modified CAV-1 DNA was digested completely with Pst Ⅰ and cloned into, plasmid pBluescript SK taking shotgun procedure. Through hybridization selection of photobiotin labeled CAV-1 DNA and Pst Ⅰ digestio...
  Published online: 5 Sep 1992
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