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Virologica Sinica, 07 (3) : 362, 1992
Detection of Potato Spindle Tuber Viroid by Polymerase Chain Reaction(PCR)
Institute of Plant Protection
CAAS. Beijing 100094
A new detection method has been developed by a new technique of polymerase chaiu reaction (PCR). According to the sequence of PSTVd, two primers were designed and synthesized with automated DNA synthesizer. The cDNA was synthesized by reverse transcription of PSTVd RNA, the cDNA was then amplified by polymerase chain reaction, the amplified products were detected by gel electrophorcsis. The results showed that the method was highly specific and the sensitivity was 0.15 pg. It was more sensitive than other m...
  Published online: 5 Sep 1992
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