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Virologica Sinica, 07 (4) : 408, 1992
Amplification and Orientation of Dengue 2 Virus Partial E Gene Insert in pUC18 by Universal Primer Directed PCR
DePartment of Microbiology
Sun Yatsen University of Medical Sciences
Guangzhou 510089
A portion of dengue 2 virus strain Haina 98 (DEN2 HN98)envelope (E1-305 nt) gene has been isolated by reverse transcription and polymerase chain reaction(RT-PCR). The plasmid pDEⅡ305 was constructed by blunt ligation of the PCR products of 305 bp fragment with SmalⅠdigested pUC18. The DEN2 E305 insert were isolated and amplified from pDEⅡ305 by universal primer directed PCRo The pUC/M13 sequencing primers (forward and reverse, Promega)and DEN2 E.specific primers(forward and reverse, Promege) and DEN2 E spec...
  Published online: 5 Dec 1992
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