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Virologica Sinica, 07 (4) : 408, 1992
Review
Amplification and Orientation of Dengue 2 Virus Partial E Gene Insert in pUC18 by Universal Primer Directed PCR
CHEN Huo-Sheng, GUO Hui-Yu, LIU Chang-Xiu, TANG Wei-Qi, SHI Song, QIU Zhong-Ren, HUANG Xiao-Jun, ZHUNG Jian
DePartment of Microbiology
Sun Yatsen University of Medical Sciences
Guangzhou 510089
Sun Yatsen University of Medical Sciences
Guangzhou 510089
Correspondence: 
(175.18KB)
Abstract
A portion of dengue 2 virus strain Haina 98 (DEN2 HN98)envelope (E1-305 nt) gene has been isolated by reverse transcription and polymerase chain reaction(RT-PCR). The plasmid pDEⅡ305 was constructed by blunt ligation of the PCR products of 305 bp fragment with SmalⅠdigested pUC18. The DEN2 E305 insert were isolated and amplified from pDEⅡ305 by universal primer directed PCRo The pUC/M13 sequencing primers (forward and reverse, Promega)and DEN2 E.specific primers(forward and reverse, Promege) and DEN2 E spec...
Key Words: PCR UniversaI Primer Dengue. Virus cDNA
Published online: 5 Dec 1992
