DePartment of Microbiology Sun Yatsen University of Medical Sciences Guangzhou 510089
Correspondence:
(175.18KB)
Abstract
A portion of dengue 2 virus strain Haina 98 (DEN2 HN98)envelope (E1-305 nt) gene has been isolated by reverse transcription and polymerase chain reaction(RT-PCR). The plasmid pDEⅡ305 was constructed by blunt ligation of the PCR products of 305 bp fragment with SmalⅠdigested pUC18. The DEN2 E305 insert were isolated and amplified from pDEⅡ305 by universal primer directed PCRo The pUC/M13 sequencing primers (forward and reverse, Promega)and DEN2 E.specific primers(forward and reverse, Promege) and DEN2 E spec...