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Virologica Sinica, 07 (4) : 426, 1992
Construction of Intermediate Vector Expressing Antisense RNA Gene for the Assembly Origin of TMV-RNA
Shanghai Institute of Biochemistry
Academia Sinica
Shanghai 200031
In this paper we have designed and constructed an antisense gene which is complementary with the assembly origin of TMV-RNA, then a intermediate vector which contains the expressing unitgconsisting of CaMV 35S pro. moter, antisense RNA gene and poly A signal, as well as the NPT-Ⅱ expressing unit was constructed, The NPT-Ⅱ is essential for the selection and transformation of plants, Thus, the successful construction of this interme diate vector will faciliate obtaining the transgenic plants.
  Published online: 5 Dec 1992
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