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Virologica Sinica, 08 (1) : 25, 1993
Review
Preparation and Application of Biotin-labelled Human Cytomegalovirus Nucleic Acid Probe
Virus Researcli Institute
Hubei Medical College
Wuhan 430071
 Correspondence:
(154.72KB)  
Abstract
A diagnostic assay has been developed to detect human cytomegalovirus (HCMV)DNA in clinical specimens with biotin-labelled DNA probe, cloned fragment of HCMV strain ADl69.The biotin-labelled probe can detect 100 pg of homologous DNA and does not hybridize with DNA from other herpesvirus or human embryonic lung cells(HEL) and Hep-2 cells. 30 umbilical cord blood and 10 urine specimens from pregnant women were examined by hybridization assay with biotin-labelled probe. Among 30 umbilical cord blood specimens,...
  Published online: 5 Mar 1993
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