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Virologica Sinica, 08 (1) : 53, 1993
Subcloning of Hemorrhagic Fever with Renal Syndrome Virus (HFRSV) Gene Fragments
The Fourth Military Medical University
Xi an 71OO32
Navy General Hospital of PLA
Beijing 100037
Based on the systematic analysis of HFRSV strain 76/118 (Hantaan virus) M and S gene with personal computer, the endonucleased fragments of tha two gene cDNA were subcloned for smaller gene probes and expression studies afterwards. The fragment containing G2 gene and the HindⅢ -BamH Ⅰ 437bp fragment of S gene were cloned into pX62, the positive recombinants of the former were selectecd by inactivation of XylE gene, a chromogenic selective marker, while the latter did not inactivate XylE gene expression, in ...
Key Words: HFRSV;  Gene clone;  XylE
  Published online: 5 Mar 1993
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