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Virologica Sinica, 08 (1) : 53, 1993
Review
Subcloning of Hemorrhagic Fever with Renal Syndrome Virus (HFRSV) Gene Fragments
The Fourth Military Medical University
Xi an 71OO32
Navy General Hospital of PLA
Beijing 100037
 Correspondence:
(188.89KB)  
Abstract
Based on the systematic analysis of HFRSV strain 76/118 (Hantaan virus) M and S gene with personal computer, the endonucleased fragments of tha two gene cDNA were subcloned for smaller gene probes and expression studies afterwards. The fragment containing G2 gene and the HindⅢ -BamH Ⅰ 437bp fragment of S gene were cloned into pX62, the positive recombinants of the former were selectecd by inactivation of XylE gene, a chromogenic selective marker, while the latter did not inactivate XylE gene expression, in ...
Key Words: HFRSV;  Gene clone;  XylE
  Published online: 5 Mar 1993
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