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Virologica Sinica, 08 (1) : 65, 1993
Detection of HCV-RNA in Plasmas of Blood Donors by Nested Double Polymerase Chain Reaction Method——And the Evaluation of Anti-HCV Antibody Test as a Blood Donation Screening Assay
Wuhan Blood Traijsfusicn Center
Wuhan 430030
Japanese Red Cross Fukuoka Blood Center
Japan 818
In this paper we reported the detection of HCV-RNA in plasma of blood donors by nested double polymerase chain reaction method using two pairs of primers located in the 5 -terminal non-coding region. We found that nested double PCR method is much better than single PCR method both in sensitivity and specificity. And we also found that only 35.2% (19/54)of anti-HCV(C100-3)positive plasma samples can be confirmed to be also PCR positve. We concluded that because the false positve rate of anti-HCV ELISA is too...
  Published online: 5 Mar 1993
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