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Virologica Sinica, 08 (1) : 84, 1993
Purification and Serology of Sweet Potato Feathery Mottle Virus
Institute of Genetics
Jiangsu Academy of Agricultural Sciences
Nanjing 210014
Sweet potato feathery mottle virus (SPEMV) was purified from infected I.Nil leaves using ultracentrifugation and CsCl step gradient centrifugations. Virus yields of 13—15 mg Per kilogram of infected Ieaf tissue were obtained. The normal particle lengths of the SPFMV was 820—860nm. The virus obtained was highly infectious and a good immunogen. Purified virus had a OD260/OD280 ratio of 1.21.
  Published online: 5 Mar 1993
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