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Virologica Sinica, 08 (1) : 102, 1993
Comparison of Four Serological Methods for Detecting CMV and TMV in the Crude Extracts of Diseased Tobacco Leaves
Department of Plant protection
Changsha 410128
Three ELISA and an SA-test methods were used to detect CMV and TMV in the crude extracts of diseased plant. For the sensitivity, Dot-ELISA method is the best with maximum dilution point of 1.28—2.56×10~(-3), followed by DSM-ELISA and SPA-ELISA (6.4×10~(-2)), SA-test is lower(0.8× 10~(-2)). For the nonspecific reaction, SPA-ELISA is lower than DSM-ELISA. When the antisera are absorbed by the extracts from the health plants, it was avoided to a great extent in Dot-ELISA and SA-test.
  Published online: 5 Mar 1993
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