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Virologica Sinica, 08 (1) : 114, 1993
Studies on PCR for Safety-detection of Rabies Virus in Samples
Department of Microbiology
Hebei Medical College
Polymerase Chain Reaction (PCR) combined with Heat-inactivation is adopted to detect of rabies virus. Using a pair of primers specific for rabies virus, reverse transcription and PCR were performed. The amplified products were visualized after electrophoresis on 2% agarose gel, stained with ethidium bromide and exposed to ultroviolet light. It is shown that:(1) A DNA fragment corresponding in size to the distance between the two primers (329 bp) could be amplified from the nucleic acid extracted from the ra...
  Published online: 5 Mar 1993
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