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Virologica Sinica, 08 (2) : 132, 1993
Induction and Enhancement of CPE in HFRS Viruses Infected Vero-E6 Cells by HEPES
National Institute for the Control of Pharmaceutical and Biological Products
Beijing 100050
We have found that HEPES could induce and enhance the CPE caused by HFRS viruses in cultured Vero-E_6 cells. The infected cells were treated with 10-100mmol/L HEPES at 2—8 days post inoculation, several hours later, polykaryocytoses, networks and vacuoles appeared in the cell cultures. It could be seen under microscope without staining. On the contrast, no CPE was found in the untreated infective Vero-E_6 control cells or treated uninfected control cells The Vero-E_6 cells infected with 76-118, L_(99), HB_(...
  Published online: 5 Jun 1993
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