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Virologica Sinica, 08 (2) : 164, 1993
Studies on Techniques of Detecting Papaya Ringspot Virus
Plant Protection Department
South China Agriculture University Guangzhou
Institute of Microbiol.
Academia Sinica
A sensitive indirect dot-ELISA was developed by using nitrocellulose membrance (NCM) instead of microplate, 3% casin as a blocking agent, horse-radish peroxidase conjugated protein A as an enzyme-labelled complex and a dilution by 100 folds of IgG from antiserum of rabbit to PRV-Ys strain as the working concentration. and was found to b workable for assessing an amount of PRV particles at ng level (ave.10ng/spot, at 37℃). A PRV-RNA cDNA molecular probe (the insert ca. 300bp) was successfully constructed wit...
  Published online: 5 Jun 1993
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