Hello guest, Register  |  Sign In
Virologica Sinica, 08 (2) : 164, 1993
Review
Studies on Techniques of Detecting Papaya Ringspot Virus
Plant Protection Department
South China Agriculture University Guangzhou
510642
Institute of Microbiol.
Academia Sinica
Beijing
 Correspondence:
(162.45KB)  
Abstract
A sensitive indirect dot-ELISA was developed by using nitrocellulose membrance (NCM) instead of microplate, 3% casin as a blocking agent, horse-radish peroxidase conjugated protein A as an enzyme-labelled complex and a dilution by 100 folds of IgG from antiserum of rabbit to PRV-Ys strain as the working concentration. and was found to b workable for assessing an amount of PRV particles at ng level (ave.10ng/spot, at 37℃). A PRV-RNA cDNA molecular probe (the insert ca. 300bp) was successfully constructed wit...
  Published online: 5 Jun 1993
Home |  Resources |  About Us |  Support |  Contact Us |  Privacy |  Powered by CanSpeed | 
Copyright © 2015 Virologica Sinica. All rights reserved