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Virologica Sinica, 08 (3) : 207, 1993
Review
Preliminary Study on the Improved Antibody Capture ELISA for Detection of EHF Specific IgE,IgA and IgG Antibodies
Zibo Second Health School
Zibo
Shandong
255015
 Correspondence:
(148.61KB)  
Abstract
In this study, an improved antibody capture ELISA method was established for the detection of EHF specific IgE, IgA and IgG antibodies. The anti-human IgE, IgA and IgG monoclonal antibodies were used as coated antibodies and Hantaan virus group specific McAb (A35, A25-1) were used as HRP conjugates. In order to simplify the procedure, shorter test time and promote the sensitivity, the EHF virus antigen and HRP-McAb were mixed up then added simultaneously. Thirty-nine EHF patient s sera were tested by this ...
  Published online: 5 Sep 1993
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