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Virologica Sinica, 08 (3) : 296-300, 1993
Studies on the Purification and Biochemical Properties of BaYMV
Shanhai Institute of Biochemistry
Academia Sinica
200031Departement of Biology
Hangzhou University
The Barley Yellow Mosaic disease is an important crop disease spread in the suburbs of Shanghai and coastal arears in East China. A purification procedure of Shanghai isolate of BaYMV was developed using the combination method of organic solvent treatment,ultracentrifugation,sucrose and CsCl densi- ty gradient centrifugation. The isolate showed the existence of two peaks in particle-length distribution (300 nm and 600 nm). SDS-PAGE of purified virus preparation demonstrated one major band of 29kd. A minor b...
  Published online: 5 Sep 1993
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