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Virologica Sinica, 08 (4) : 310-315, 1993
Rapid Detection of HBV-DNA by Using Biotinylated Oligonucleotide Probe
Wuhan Institute of Virology
Academia Sinica
Wuhan 430071
A rapid method for detection of HBV-DNA was developed. This assay was based on the hybridization of biotinylated oligonucleotide probe to target DNA immobolized on nitrocellulose membrane which was then incubated with avidin-alkaline phosphatase conjugator and measured with chromogenic substrates. The practicality of the method was investigated. It was found that the method was fast (4hr),sensitive(1-10pg)and expedient for laboratory and clinical detection of HBV-DNA.
  Published online: 5 Dec 1993
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