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Virologica Sinica, 08 (4) : 385-389, 1993
Review
The High-level Expression of Beet Necrotic Yellow Vein Virus Coat Protein Gene in Escherichia coli
National Laboratories for Agrobiotechnology
Beijing Agricullural University
Beijing 100094
 Correspondence:
(139.69KB)  
Abstract
The temperature-inducible expression vector containing the beet necrotic yellow vein virus(BNYVV)isolate NM coat protein gene was constructed and transferred into E. coli DH5 α. High-level expression of the specific protein was achieved by temperature induction. The results of SDSPAGE and Western blotting show that the expression product which accumulates 19.5% of the total cellular proteins estimated by Shimadzu CS-910 scanning is 21kd BNYVV coat protein.
Key Words: BNYW;  Coat protein gene;  Expression
  Published online: 5 Dec 1993
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