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Virologica Sinica, 09 (1) : 18, 1994
Expression of HCV Core Protein in E.c0fi and Its Application
Nanjing Military Medical Research Institute,Nanjing 210002
The cloned HCV core protein gene was inserted into the plasmid vector pJLA502 at EcoRI site,and the recombinant plasmid was transformed into E,coli DH_(5a),After inducing 5hrs at 42℃,SDS-PAGE analysis showed that the expressed core protein is twenty percent of total protein of E.coliDH5a;the purified core protein by Sephadex G200 gel filtration and Sepharose-4B-IgG affinity chro-matography has excellent antigenicity and specificity,and is good for detecting anti-HCV.
  Published online: 5 Mar 1994
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