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Virologica Sinica, 09 (1) : 18, 1994
Review
Expression of HCV Core Protein in E.c0fi and Its Application
Nanjing Military Medical Research Institute,Nanjing 210002
 Correspondence:
(198.43KB)  
Abstract
The cloned HCV core protein gene was inserted into the plasmid vector pJLA502 at EcoRI site,and the recombinant plasmid was transformed into E,coli DH_(5a),After inducing 5hrs at 42℃,SDS-PAGE analysis showed that the expressed core protein is twenty percent of total protein of E.coliDH5a;the purified core protein by Sephadex G200 gel filtration and Sepharose-4B-IgG affinity chro-matography has excellent antigenicity and specificity,and is good for detecting anti-HCV.
  Published online: 5 Mar 1994
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