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Virologica Sinica, 09 (1) : 70, 1994
The Application of Rapid HEPES-plaques-forming Test(RHPFT)to Rabies Virus Titration
Institute of Shanghai Biological Products
According to the phenomenon that HEPES could enhance CPE in rabies virus infected cell and accelerate the plaques formation,the virulence of rabies virus(RFD strain)was detected with 3 differentmethods includig RHPFT,MC-plaques test and mollse i.c,test.The results showed that the sensibility of 3 detecting methods was similar but RHPFT was more rapid and simple than the latter two,Theresult of RHPFT methed could be obtained earlier about 6-7 days than MC-plaques test which everused as a routine assay in rabies virus titration.RIlPFT could also be used for rabies neutralizing anti-body assay.
  Published online: 5 Mar 1994
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