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Virologica Sinica, 09 (1) : 70, 1994
Review
The Application of Rapid HEPES-plaques-forming Test(RHPFT)to Rabies Virus Titration
Institute of Shanghai Biological Products
 Correspondence:
(106.89KB)  
Abstract
According to the phenomenon that HEPES could enhance CPE in rabies virus infected cell and accelerate the plaques formation,the virulence of rabies virus(RFD strain)was detected with 3 differentmethods includig RHPFT,MC-plaques test and mollse i.c,test.The results showed that the sensibility of 3 detecting methods was similar but RHPFT was more rapid and simple than the latter two,Theresult of RHPFT methed could be obtained earlier about 6-7 days than MC-plaques test which everused as a routine assay in rabies virus titration.RIlPFT could also be used for rabies neutralizing anti-body assay.
  Published online: 5 Mar 1994
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