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Virologica Sinica, 09 (2) : 125-129, 1994
Detection of Human Cytomegalovirus in Neonatal Hepatitis by Nested PCR
Department of biology ,Nanjing Railary Medical College ,Nanjing 210009
ixty-one children patients with neonatal hepatitis were investigated for human cytomesalovirus(HCMV)by single PCR and nested double PCR.of the 61 patients,33(54.1%)were positive forHCMV by single PCR,whereas 47(77.0%)were positive by nested double PCR.This showed that nest-ed double PCR is more sensitive than single PCR,and also revealed that HCMV can be one of the important agents for neonatal hepotitis.In addition,its advantage of high sensitivity and specificty com-bined with more rapid detection suggests that this precedure can be a potential diagnostic and epidemio-logical tool.
  Published online: 5 Jun 1994
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