Hello guest, Register  |  Sign In
Virologica Sinica, 09 (2) : 130-137, 1994
Review
PCR Amplification CloningProtein Gene and Location of Major Capsid Of Baculovirus
Institute of Hology ,Wuhan University ,Wuhan 430072
 Correspondence:
(250.70KB)  
Abstract
he major eapeid protein gene(vp39)of Autographa californica nuclear polyhedrosis virus(AcNPV)was amplified successfully by PCR technique and cloned into pBluescript SK(+).With pure AcNPVvp39 gene as a probe,the major capeid protein gene(vp39)of Leucania seperata nuclear polyhedrosisvirus has been leeated on PstI-F.BamHI-C,EcoRI-C,E,XhoI-D,I,EcoRV-H,X fragnients by Southern blot.Besides amplification of the predicted 1406bp fragment including intact major capsid proteingene of AcNPV using XhoI and HindⅢ-digested AcNPV DNA as a teniplate.a ca.400 bp fragmenthas been amplified.In this paper we disscused the PCR amplification of ca.400 bp fragment of bothspecificity and nonspocificity.
  Published online: 5 Jun 1994
Home |  Resources |  About Us |  Support |  Contact Us |  Privacy |  Powered by CanSpeed | 
Copyright © 2015 Virologica Sinica. All rights reserved