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Virologica Sinica, 09 (2) : 151-156, 1994
Preparation of High Titer Antiserum to Swee!Potato Feathery Mottle Virus(SPFMV)
Departmnet of Inner Mongotin Universityl ,Huhehaote 01002I
weet potato feathery mottle virus(SPFMV)was inoculated to I.selosa to propagare by graftingand was purified wirh 0.2mol/L pH7.2 PBK.Further purification of SPFMV was conducted by sucrose cushion and sucrose density gradient centrifugation.Purified SPFMV had a OD260/280 ratio of1.25.Rilbbit was immuned with purifled viruses to prepare antiSerum.Antisera produced to purifiedvirus had a titer of 1:1096 in microprecipitin and ring precipitin tests.SPFMV in leaves of sweet potato and I.selosa were detected respectively by indirect enzyme-linked immunosorbent assay(ELISA)onnitreeellulose membranes.
  Published online: 5 Jun 1994
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