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Virologica Sinica, 09 (2) : 151-156, 1994
Review
Preparation of High Titer Antiserum to Swee!Potato Feathery Mottle Virus(SPFMV)
Biology  
Departmnet of Inner Mongotin Universityl ,Huhehaote 01002I
 Correspondence:
(189.32KB)  
Abstract
weet potato feathery mottle virus(SPFMV)was inoculated to I.selosa to propagare by graftingand was purified wirh 0.2mol/L pH7.2 PBK.Further purification of SPFMV was conducted by sucrose cushion and sucrose density gradient centrifugation.Purified SPFMV had a OD260/280 ratio of1.25.Rilbbit was immuned with purifled viruses to prepare antiSerum.Antisera produced to purifiedvirus had a titer of 1:1096 in microprecipitin and ring precipitin tests.SPFMV in leaves of sweet potato and I.selosa were detected respectively by indirect enzyme-linked immunosorbent assay(ELISA)onnitreeellulose membranes.
  Published online: 5 Jun 1994
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