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Virologica Sinica, 09 (3) : 206, 1994
Demonstration of infectious mature HIV-1 carrying pre-viral DNA by PCR
National Institute for the control parmocatical and biological products,beijing 100050
Infectious mature human immunodeficiency virus type 1 (HIV-1) carruig pre-virus DNA was evidenced by pelpoerase chani reaction(PCR).When the samples were treated with lytic buffer,the result of PCR should be positive;however when PBS was added uistead of lytic buffer,the DNA wasnot detected. The DNA was sensitive to DNase treamient after lysisof viral particles.However,theDNA was not seasitive to DNase treatinent for the intact viral paricles.The DNA was not detected after the CD_4 receptor of the C8_(166) cells were covered by anti-CD_4 receptor monoclonal antibody(Leu3a),and the virus could not absorb to the cells.Furthermore,the number of DNA was correlativewith virus titer.This evidence supported the HIV-I virus carrying pre-virus DNA.
  Published online: 5 Sep 1994
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