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Virologica Sinica, 09 (3) : 217, 1994
Review
Cloning of restriction fragments from Agrotis segetum granulosis virus DNA and localization of the gran ulin gene
Wuhan Institute of Virology,CAS,Wuhan 430071
 Correspondence:
(143.08KB)  
Abstract
Agrotis segetum granulosis virus (AsGV) is an important biolosical control agent for A.segelum.Several PstI fragments from AsGV were cloned into pUC19.Under the very low strinsency(20% formamide,37℃ ),we have leealized the granulin sene on BglII-S or T,EcoRI-A,PstI-A or B frag.ments of AsGV DNA,by using the 32P-labelled polyhedrin gene of Buzura suppressaria nuclear polyhedrosis virus (BsNPV)as a prcbe.
  Published online: 5 Sep 1994
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