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Virologica Sinica, 09 (3) : 217, 1994
Cloning of restriction fragments from Agrotis segetum granulosis virus DNA and localization of the gran ulin gene
Wuhan Institute of Virology,CAS,Wuhan 430071
Agrotis segetum granulosis virus (AsGV) is an important biolosical control agent for A.segelum.Several PstI fragments from AsGV were cloned into pUC19.Under the very low strinsency(20% formamide,37℃ ),we have leealized the granulin sene on BglII-S or T,EcoRI-A,PstI-A or B frag.ments of AsGV DNA,by using the 32P-labelled polyhedrin gene of Buzura suppressaria nuclear polyhedrosis virus (BsNPV)as a prcbe.
  Published online: 5 Sep 1994
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