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Virologica Sinica, 09 (3) : 272, 1994
Use of Polymerase Chain Reaction for the Infection with Herpes Simplex Virus in Chronic Cervicitis
Nanjing Medical University
PCR was adapted to detect HSV-DNA sequence in samples from 72 petients with clironic cervici-tis and 30 healthy cotrols,and compared with McAb-ELISE and virus isolation(VI).The plsitive rateof HSVby these three methods was 28.4% 22.5% 20.6% respotively,and coinciuent rates a-mong threelmired methforwere allover 90%,It isshown that PCR cpmbined withcell lysis isa fast,effective and relle ble methed to detect HSV in the cervix,It can detect not only very low quantityHSV-DNA,but also inactive and latent HSV.HSV postivity rate of chronic cervicitis is markedly high-er than healthy control(P<0.05).
  Published online: 5 Sep 1994
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