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Virologica Sinica, 10 (2) : 120, 1995
Molecular Cloning and Sequence of the HCV NS1 Genomic Region
Department of Infectious Diseases
Sun Yat-sen University of medical Sciences
Guangzhou 510630
The NS1 genomic region of the hepatitis C virus(HCV),derived from the serum of a patient withchronic NANB hepetitis in Guangdong province,was cloned and sequenced.HCV RNA extracted bythe powder-adsorption procedure was converted to cDNA by everse transcription with random primer.Polymerase chain reaction was performed with the primers which span the NS1 region,The amplifiedproducts were separated by electrophoresis on a low melting-point agarose gel and a portion of gel,cor-respondlng to the expected size,w...
  Published online: 5 Jun 1995
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