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Virologica Sinica, 10 (2) : 131, 1995
Review
Chemical Synthesis of an Oligonucleotide Probe and Its Detection of JEV
Institute of Molecular Biology
The Fourth Military Medical University
Xi an 710032
 Correspondence:
(121.16KB)  
Abstract
probe(19 oligonucleotides)for JEV corresponding to the sequence from 11 to 29 nt of the 3 end of Japanese encephalitis virus genomic RNA was designed and syn thesized with ABI 381A DNAsynthesizer,The oligonucleotide probe was labeled with[α-(32)p]using the Klenow fragment.Thespecific radioactivity of the probe is 8.10-8.86x107cpm/μgDNA.Hybridization with this probeshowed that lpg of target DNA,or the viruses harvested from the C6/36 cell culture at 6-12 hoursafter infection with JEV,Could be detected.
  Published online: 5 Jun 1995
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