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Virologica Sinica, 10 (2) : 131, 1995
Chemical Synthesis of an Oligonucleotide Probe and Its Detection of JEV
Institute of Molecular Biology
The Fourth Military Medical University
Xi an 710032
probe(19 oligonucleotides)for JEV corresponding to the sequence from 11 to 29 nt of the 3 end of Japanese encephalitis virus genomic RNA was designed and syn thesized with ABI 381A DNAsynthesizer,The oligonucleotide probe was labeled with[α-(32)p]using the Klenow fragment.Thespecific radioactivity of the probe is 8.10-8.86x107cpm/μgDNA.Hybridization with this probeshowed that lpg of target DNA,or the viruses harvested from the C6/36 cell culture at 6-12 hoursafter infection with JEV,Could be detected.
  Published online: 5 Jun 1995
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