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Virologica Sinica, 10 (2) : 161, 1995
Construction of a cDNA Libray of the Bovine Viral Diarrhea Virus Genome
Wuhan Institute of Virology
Academia Sinica
Wuhan 430071The Academy of Science of Animal Husbandry of Inner Mongol Autonomous Region
Huhehot 010030
ovine viral diarrhea virus(BVDV)InL genomic RNA was used as a template to synthesizeDNA by reverse transcriptase.The ds cDNA was synthesized by RNase H-DNA polymerase,and waslsetrted into the Pst I site of the pUC8 vector by dC-dG homopolymeric tailing and ligation.After beig transformed to E.coli JM101,the recombinants were screened by in situ hybridization with γ- ̄(32)P-ATP labeled purified BVDV RNA,Restrict ion enzyme ana lysis sho wed that the largest cDNA insertwas 12kb-13kb,it almust covered the BVDV...
  Published online: 5 Jun 1995
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