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Virologica Sinica, 10 (2) : 169, 1995
Rabbit haemorrhagic disease virus:Isolation of messenger RNA and identification of its activity
poly(A)tRNAs enriched with rabbit haemorrhagic disease virus(RHDV)proteins were isolatedfrom RHDV-infected rabbit liver tissue by polysome immunoprecipitation and purified by oligo(dT)-cellulose chromatography.The purified poly(A)tRNA was identified through denatural agarose elec-trophoresis Six single narrow bands,5.01,3.62,2.40,1.82,1.22 and 0.95 kb respectively,were vi-sualized.The in vitro translation products of viral mRNAs have been demonstrated by SDS-PAGE and immunoblotting analysis,proteins with molecular weight of 64,59,54,51,29,24 and 20kD wereshown RHDV-specific.
  Published online: 5 Jun 1995
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