Department of Veterinary Microbiology and Immunology. Nanjing Agricultural.University Nanjing 210095Cao Jie Chen Qingxu an Song DesiuInstitute of Develop Mental Biology Academia Sinica Beijing 100080
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(80.08KB)
Abstract
Poly(A) ̄+RNAs enriched with rabbit haemorrhagic disease virus(RHDV)proteins were isolatedfrom RHDV-infected rabbit liver tissue by polysome immunoprecipitation and purified by oligo(dT)-cellulose chromatography.The purified poly(A) ̄+RNA was identified through denatural agarose elec-trophoresis Six single narrow bands,5.01,3.62,2.40,1.82,1.22 and 0.95 kb respectively,were vi-sualized.The in vitro translation products of viral mRNAs have been demonstrated by SDS-PAGE and immunoblotting analysis,proteins with ...