Rabbit Haemorrhagic Disease Virus:Isolation of Messenger RNA and Identification of Its Activity

GONG Xiao-Meng; DU Nian-Xin; CAO Jia; CHEN Qing-Han; SONG De-Xiu

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Virologica Sinica, 10 (2) : 177, 1995

Review

Rabbit Haemorrhagic Disease Virus:Isolation of Messenger RNA and Identification of Its Activity

GONG Xiao-Meng, DU Nian-Xin, CAO Jia, CHEN Qing-Han, SONG De-Xiu

Department of Veterinary Microbiology and Immunology. Nanjing Agricultural.University
Nanjing 210095Cao Jie
Chen Qingxu an Song DesiuInstitute of Develop Mental Biology
Academia Sinica
Beijing 100080

Correspondence:

(80.08KB)

Abstract

Poly(A)￣+RNAs enriched with rabbit haemorrhagic disease virus(RHDV)proteins were isolatedfrom RHDV-infected rabbit liver tissue by polysome immunoprecipitation and purified by oligo(dT)-cellulose chromatography.The purified poly(A)￣+RNA was identified through denatural agarose elec-trophoresis Six single narrow bands,5.01,3.62,2.40,1.82,1.22 and 0.95 kb respectively,were vi-sualized.The in vitro translation products of viral mRNAs have been demonstrated by SDS-PAGE and immunoblotting analysis,proteins with ...

Key Words: Rabbit haemorrhagic disease virus; Messenger RNA; Polysome immunoprecipitation; in vit ro translation

Published online: 5 Jun 1995