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Virologica Sinica, 10 (2) : 177, 1995
Review
Rabbit Haemorrhagic Disease Virus:Isolation of Messenger RNA and Identification of Its Activity
Department of Veterinary Microbiology and Immunology. Nanjing Agricultural.University
Nanjing 210095Cao Jie
Chen Qingxu an Song DesiuInstitute of Develop Mental Biology
Academia Sinica
Beijing 100080
Nanjing 210095Cao Jie
Chen Qingxu an Song DesiuInstitute of Develop Mental Biology
Academia Sinica
Beijing 100080
Correspondence: 
(80.08KB)
Abstract
Poly(A)￣+RNAs enriched with rabbit haemorrhagic disease virus(RHDV)proteins were isolatedfrom RHDV-infected rabbit liver tissue by polysome immunoprecipitation and purified by oligo(dT)-cellulose chromatography.The purified poly(A)￣+RNA was identified through denatural agarose elec-trophoresis Six single narrow bands,5.01,3.62,2.40,1.82,1.22 and 0.95 kb respectively,were vi-sualized.The in vitro translation products of viral mRNAs have been demonstrated by SDS-PAGE and immunoblotting analysis,proteins with ...
Key Words: Rabbit haemorrhagic disease virus; Messenger RNA; Polysome immunoprecipitation; in vit ro translation
Published online: 5 Jun 1995
