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Virologica Sinica, 10 (3) : 228, 1995
Mapping the immunoreactive epitope encoding sequence of HPV 6b L20RF
Department of Microbiology, Shandong Medical University
PV contains two late open reading frames. T he L_2 protein encoded by L_2 ORF possesestype-specific antigenicity. To map the immunoreactive epitope of HPV 6b L_2,a L_2 expressionplasmid p6bL_2 NX was used.Plasmid p6b L_2 NXK was made by adding K pnI linker intopolylinker of p6bL_2 NXK. Unidirectional 3’ to 5’deletions were made in the HPV 6b L_2 ORFinsert of p6b L_2 NXK by using exonuclease Ⅲ and nuclease S_1.The deleted plasmids ex-pressed a set of carboxy to amino terminus truncated fusion proteins. Region containing theimmunoreactive epitope was mapped by determing retained reactivity of the deleted fusionproteins with sera in Western immunoblot assays. The related plasmids were sequuenced.The immunoreactive epiutope was located at 5481-5506 nts region,and the correspondingpepude was EPGINPTQ.
  Published online: 5 Sep 1995
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