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Virologica Sinica, 10 (3) : 254, 1995
Studies on the Detection of Cueum ber M osaie Virus in M ieroprogation of Banana
Department of Plant Protection,South China Agricultural University, Guangzhou 510642
The present study established the direct antigen coating form for indirect ELISA (DAC-FLISA)for the detection of cucumber mosaic virus(CMV)in banana tissue culture. With aproper phosphate buffer solution,i.e.0. 1mol/L phosphate buffer(pH7.2,including 1%sodium diethyldithiocarbaniate) for preparing the crude plant sap to be detected,it was possi-ble to increase the sensitivity of DAC一ELISA to a dilution of plant sap at a rate of 1/5120.Atotal of 1 287 samples of productive material for banana tissue culture were detected forCMV.2.8%of them were found to be infected with CMV.
  Published online: 5 Sep 1995
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