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Virologica Sinica, 10 (3) : 261, 1995
Purification of Rabbit Haemorrhagie Disease Virus from Counter Immunoelectrephoresis
Medical College of Animals, Nanjing Agricultural University
HDV could be moved to the negative electrode by electrophoresis in the condition ofpH8.6 ,and this was confirmed by immunoelectron microscopy observation. The RHDV im-munocomplex was purified by counter immunoelectrophoresis(CIE)form liver tissue of rab-bits died of RHD and used for the analysis of RHDV structural polypeptide by immunoblot-ing.The RHDV nucleic acid was extracted from RHDV immunocomplex and labelled withDigoxigenin-dUTP. The labelled nucleic acid probe with high senseitivity and specificity wasable to hybrideized with RHDV nucleic acid,RHDV 2. 2kb and4. 8kb cDNA fragments in dotblot analysis.
  Published online: 5 Sep 1995
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