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Virologica Sinica, 10 (3) : 261, 1995
Review
Purification of Rabbit Haemorrhagie Disease Virus from Counter Immunoelectrephoresis
Medical College of Animals, Nanjing Agricultural University
 Correspondence:
(106.51KB)  
Abstract
HDV could be moved to the negative electrode by electrophoresis in the condition ofpH8.6 ,and this was confirmed by immunoelectron microscopy observation. The RHDV im-munocomplex was purified by counter immunoelectrophoresis(CIE)form liver tissue of rab-bits died of RHD and used for the analysis of RHDV structural polypeptide by immunoblot-ing.The RHDV nucleic acid was extracted from RHDV immunocomplex and labelled withDigoxigenin-dUTP. The labelled nucleic acid probe with high senseitivity and specificity wasable to hybrideized with RHDV nucleic acid,RHDV 2. 2kb and4. 8kb cDNA fragments in dotblot analysis.
  Published online: 5 Sep 1995
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