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Virologica Sinica, 10 (4) : 323, 1995
Cloning of parvovirus H-1 NS partial gene by PCR
Two modified primers,△P3 and △P4,were designed and,for each of them,two mutatedbases were included.A fter PCR amplification, the DNA fragment contains a restriction endonu-clease recognition site,BamHⅠ or Hind Ⅲ at its ends,Cloning of the parvovirus H-1 NS-1 partialgene was conducted by double digestion uf the aniplified DNA fragement with endonucleases,andits presence in the inserted fragement was confirmed by DNA sequence analysis.The techniquedescribed here provides the basis for the quality control of H-1 propagation,and for the studies ofoncosuppressive action of H-1 NS-1 protein and of transcription and expression of NS-1 gene incancer cells and normal tissues.
  Published online: 5 Dec 1995
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