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Virologica Sinica, 11 (1) : 45, 1996
Study on biologic activity for membrane and enzyme of norma} bone marrow ceils infected with epidemic hemorrhagic fever virus
Union Hospital,Tongji Medical Uniwersity,Wuhan 430022
sing DPH fluorescence probe,the membrane of normal bone marrow cells infected withepidemic hemorrhagic fever virus(EHFV)was labeled.The membrane lipid fluidity could becounted according to change of value of fluorescence polarization.At the same time,using cyto-histochemical staining quantitative method,the cellular ACP and SDH were detected.The resultsshowed:(1)The Lymphocyte,monocyte and granulocyte were observed dynamically after cul-tured 1,6,24,72 hours.The membrane lipid fluidity of infected cells appeared obviously low-There were significant differences statistically(p<0.05-0.01)(2)By cytohisto-chemical stain-ing and microspectrophotometer quantitating,before cultured 72 hours,ACP and SDH in infectedcells raised.However,after 120 hours,their values were decreased Also,there were significant(P<0.05).It could be related with virus invasion and damage degree of cellular function.
  Published online: 5 Mar 1996
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