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Virologica Sinica, 11 (1) : 84, 1996
AA-ELISA for Detection of Barley Resistance to BYDV
Shanxi Institute of Plant protection-Yangling,Shanxi 712100
wo viruliferous Rhopalosiphum padi(BYDV)can be detected by indirect enzyme-linkedimmunosorbent assaay(ELISA).Based on that result,a Aphid Acquisition-Enzyme-linked im-munosorbent assay(AA-ELISA)has been established for identification of barley resistance toBYDV.The OD value of AA-ELSA showed that the barley variety 877-16 carrying YD2 genewas significantly different from that of susceptible variety Manley.No significant difference wasfound between 877-16 and Manley when they were detected directly by ELISA at the same leave.
  Published online: 5 Mar 1996
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