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Virologica Sinica, 11 (2) : 120, 1996
The establishment and evaluation of a method for detecting anti-HEV using peptides
302 hospital of PLA
Mostly employing recombinant antigen,indirect enzyme linked immunosorbent assay is usedto detect HEV infection.Since synthetic peptides can be synthetised easily and have less nonspe-cific structures,they have been extensively used in the laboratory diagnosis of viral diseases.Basedon the reported nucleotide sequence of HEV genome and conservatism and hydrophilicity of aminoacid sequence,two synthetic peptides from ORF2 and ORF3 were prepared and used to developean EIA for detecting Anti-HEV,Detected 264 clinical specimens,the coincident rate for positivewas 97.75 % and for negative 99.43%,as comparing with DBL reagent(Singapore).Thismethod has a high sensitivity,specificity safety and speed.It is an ideal method for detection ofHEV infection and survey of HEV epidemiology.
  Published online: 5 Jun 1996
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