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Virologica Sinica, 11 (2) : 164, 1996
Construction of rice expression vector including antisense ribozyme and partial sequence of repli- case in rice dwarf virus
Institute of Microbiology ,Chinese Academy of Sciences
he whole sequence of RDV S1 fragment and partial sequence(S5Ⅲ)of RDV S5 fragmentwere synthesized and amplified by the method of reversed transcription-polymerase chain reaction(RT-PCR).Each amplified product was then cloned into pGEM7Zf(+)or pUC19 at the Smalsite,and sequenced.Based on these results,a hammer-head ribozyme sequence was introduced intoantisense chain of S5Ⅲfragment to construct antisense ribozyme gene S5ⅢR,Rice transformationvector pROK-S1-1' and pROK-S5ⅢRwere constructed by inserting S5ⅢRgene and 5'-end se-quence of S1 fragment(S1-1')into plant expression vector pROK Ⅱ.
  Published online: 5 Jun 1996
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