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Virologica Sinica, 11 (3) : 284, 1996
Review
Detection of pseudorabies virus by biotinylated DNA probe
Sichuan Agricultural University,Yaan Sichuan Province 625014
 Correspondence:
(78.09KB)  
Abstract
dot blot hybridization method was developed to detect pseudorbies virus DNA in infectedcultured cells.Seven strains of pseudorabies virus were detected by hiotinylated pseudorabies virusFa DNA probe,and recomhinant plasmid pPB11DNA(BamHI-11 fragment of PRV Fa was clonedbetween the BamHI sites of plasmid pBR322)probe.PRV Fa DNA probe could detect 10 pg nu-cleic acid in the samples. The nucleic acid hybridization method is sensitive and specific for diag-nosing pseudorabies virus.
  Published online: 5 Sep 1996
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