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Virologica Sinica, 11 (3) : 287, 1996
Amplification of the Intact Gene Coding Epstein-Barr Virus-Specific tIIym idine Kinase
Department of Biochemistry.Sun-Yet University of Medical Sciences,Guangzhou 510089
Cancer Research InStitute,Sun-sen University of Medical&iences.Guangzhou 510060
Based o1"1 Epstein—Barr virus(EBV)DNA (B95—8)genome sequenc3e data and the strut—ture BXLFlopen readingframe codingforEBV ~hymidinekinase(TK),a pairofPCR pr/mers was designed and svnthesized Template DNA was extracted rapidly from B95—8 calIs harboring EBV bv using guanidinium isothioeyanate(GuSCN)and silica∞urse(cs).A 1843bp A frag—ment containing intact EBV TK gene was amplified by polymerase chain reaction.The PCR prod—uct was verified by cleaving with NcoI
  Published online: 5 Sep 1996
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