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Virologica Sinica, 11 (4) : 369, 1996
Review
Study on ELISA method for detecting specific circulating immune complexes in sera of patients with HFRS
Zibo Second Health School,Zibo,Shandong Province 255015
 Correspondence:
(88.27KB)  
Abstract
new ELISA method was established for detecting specific IgG,IgM,IgA type circulatingimmune complexes(CIC)in sera of patients with Hemorrhagic Fever with Renal Sydrome(HFRS).The rabbit anti-HFRS virus antibody was used as coated antibody and anti-humanIgG,IgM,IgA monoclonal antibodies were used as HRP conjugates.The results showed that thedetected rates of specific IgG,IgM,IgA types CIC in 73 acute phases(1 ̄8days after illness)were 69.49%,69.86% and 56.16% respectively,in 54 convalescence phases(10 ̄28 days afterillness)were 90.74%,85.19% and 75.93% respectively,28 sera in 1 ̄3 years after illness wereall 0.00%,36 serum specimens from normal and hepatitis B patients were all negative.The developed method showed highl specificity and sensitirity as well as reproducibility(CV:IGG12.07%,IgM6.29% and IgA12.89%)for the detection of HFRS specific CIC.
  Published online: 5 Dec 1996
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