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Virologica Sinica, 12 (4) : 322-328, 1997
Review
Expression of Hantaan Virus Envelope Glycoproteins G1 and G2 in E .coli with Differential Expression Vector
Tangdu Haspital ,The Fourth Military Medical University ,Xi'an 710038,China
 Correspondence:
(176.77KB)  
Abstract
For increasing the expression level of Hantaan vitus envelope glycoproteins GI and G2 in E.coli, the expression vector pKK223-3 and pGEX-4T -1 were used for  constructingGl and G1 expression plasmids, pGEX-4T-l  was a fusion-protein expression vector phsmid.The results showed that the expression levels of hantaan virus envelope glycoproteins G1 a nd G2 w ith differential expression vector plasmids were riot ohvions different.The exprssion levels of the portion of GI andG2 proteinswere about the same with the complete G1 and G2.
  Published online: 5 Dec 1997
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