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Virologica Sinica, 12 (4) : 322-328, 1997
Expression of Hantaan Virus Envelope Glycoproteins G1 and G2 in E .coli with Differential Expression Vector
Tangdu Haspital ,The Fourth Military Medical University ,Xi'an 710038,China
For increasing the expression level of Hantaan vitus envelope glycoproteins GI and G2 in E.coli, the expression vector pKK223-3 and pGEX-4T -1 were used for  constructingGl and G1 expression plasmids, pGEX-4T-l  was a fusion-protein expression vector phsmid.The results showed that the expression levels of hantaan virus envelope glycoproteins G1 a nd G2 w ith differential expression vector plasmids were riot ohvions different.The exprssion levels of the portion of GI andG2 proteinswere about the same with the complete G1 and G2.
  Published online: 5 Dec 1997
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