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Virologica Sinica, 13 (1) : 44, 1998
Detection of hemorrhagic fever with renal syndrome virus RNA in chigger mite by in situ hybridization
Detection of Hemorrhagic Fever with Renal Syndrome Virus RNA
in Chigger Mite by in situ Hybridization
In order to study the distribution and position of HFRSV in chigger mite, in situ hybridization was used for detection of hemorrhagic fever with renal syndrome virus (HFRSV) RNA.The results showed that positive rate of HFRSV by in situ hybridization was higher than that by IFAT. Positive signals were observed mostly in belly cells of larva and nymph. The positions were probably ovary cells and midgut. A few positive signals were seen in foreleg and middle leg cells also. The positive signals of HFRSV in nymph were much more than in larva. It demonstrated that HFRSV can be transmitted by transstadial and proliferate in mites.
  Published online: 5 Mar 1998
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