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Virologica Sinica, 13 (1) : 69, 1998
Preparation and Application of the New Type Hepatitis E Virus Diagnostic Kit
Center for diagnostic Reagents,302nd Hospital of PLA,Bejing 100039
Solid phase enzyme immunoassay (EIA) kit was developed for detecting anti HEV IgG by using synthetic peptide from the open reading frame (ORF)3 and recombinant antigens from ORE2.When the kit was applied to detect HEV antibodies in sera of clinical patients the results were quite consistent with the HEV diagnostic kit from Genlabs.The consistent rate reached 100% (60/60).CV of three lot kit was less than 10%.The kit was stable for 8 months at 4℃or for 4 days at 37℃.The kit was used to detect anti HEV IgG among clinically different patients with acute Non A,Non B,Non C hepatitis,Hepatitis A,Hepatitis B,Hepatitis C and normal human group,the positive rates were 63.2%,13.4%,8.3%,6.6%,2.9%, respectively. The date indicated that the HEV EIA kit is sensitive,specificic and stable,may be used to detect antibody of Hepatitis E Virus among patients and normal human group.
  Published online: 5 Mar 1998
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