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Virologica Sinica, 13 (1) : 100, 1998
Research Article
Nested RT-PCR for Detection of Mumps Virus RNA in the Clinical Specimens
Institute of Biological Products, Lanzhou
Seventy two clinical specimens of saliva,throat swabs and urine collected from clinical mumps cases were examined by the nested RT PCR, 69 (86.1%) of them were positive. The results showed that the positive rate of the urine specimens (25%) was significant different (P<0.005) from that of the saliva (100%) and the throat swab (96.6%) specimens.The Enders strain of mumps virus was used as the positive control and measles virus,new castle disease virus,influenza viruses (A1,A3,B) and rotavirus were used as the negative controls for confirming the specificity of the method.The sensitivity test of the method indicates that at least 1TCID 50 mumps virus or 1~10pg DNA of cloned SH gene plasmid can be detected.This method can be used for clinical detection of the mumps virus with high sensitivity and specificity.
  Published online: 5 Mar 1998
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