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Virologica Sinica, 13 (2) : 138, 1998
Cloning of human interleukin 18 gene and its expression in E. coli
Institute of Basic Medical Sciences,Academy of Military Medical Scieces.Beijing 100850 Hebei Provincial Hygiene and Anti-epidemic Station,Baoding 071D00
Human interleukin 18 gene was isolated from human liver tissue by reverse transcription polymerase chain reaction.hIL 18 gene was cloned into plasmid pGEM T(Easy)and pBV220.The gene was sequenced and showed no difference with that reported and expresed in E.coli in the form of inclusion bodies.The recombinant protein amounts to 27.2% of total bacterial protein.
  Published online: 5 Jun 1998
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