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Virologica Sinica, 13 (2) : 159, 1998
Detection of the Group B Rotavirus with the Probe 0f 0lig0nucle0tides Labelled with Biotin
Animal Biotechnology Laboratory,Xinjiang Academy of Animal Science,Urumqi 830000
According to segment 3 sequence of group B rotavirus (GBRV)IDIR strain the 23 bp oligonucleotide was synthesized and labelled with biotin.The probe was purified by HPTLC.Its sensitivity is up to 10pg by a colorimetric reaction.The probe can detect at least 100pg target seuqence of GBRV and does not hybridize to Group A and Group C rotavirus.The results showed that the probe has high specificity and sensitivity.It can be used to detect and identify GBRV,and also to research the genetic relativity of GBRV gene mutation and rearrangement.
  Published online: 5 Jun 1998
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