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Virologica Sinica, 13 (4) : 350, 1998
Bioassay of the multiple Autograph californica nucleopolyhedrovirus (AcMNPV) pesticides
NanjingAgrictdtural College,Nanjing 210038
The multiple with other biological agent Autographa californica nucleopolyhedrovirus (AcMNPV) pesticide, which is widely used to control Spodoptera exigua and Prodenia litura as well as Artogeia rapat, Hellula untalis, Plutella xylostella , was selected. Tested with 2nd instar S. exigua larvae, the virulence of AcMNPV A strain amplified in S.exigua larvae is higher than that of AcMNPV B strain in Sf9 cells. LD 50 of AcMNPV B (1949.4 PIB/Larva) is 14.79 times more than AcMNPV A (131.8PIB/Larva).The ratio of LD 50 between AcMNPV A (LD 50 =168.84-240.36 PIB/Larva) and AcMNPV A+Bt (LD 50 =1.4329-128.91 PIB/Larva) is 118-1.5 while the ratio between AcMNPV A and AcMNPV A+Bt+PlNPV (LD 50 = 21.82 -21.4 PIB/Larva) is 7.7-9.5. Tested with 2nd instar P.litura larvae, the ratio of LD 50 between PlNPV (LD 50 =16.02-53.53 PIB/Larva)and PlNP V+Bt(LD 50 =2.9-7.76 PIB/Larva ) is 5.5-6.9 while the ratio between PlNPV and PlNPV+Bt+AcMNPV A (LD 50 =3.16-15.469
  Published online: 5 Dec 1998
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