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Virologica Sinica, 14 (2) : 106-109, 1999
Propagation Characteristics of Coxsackie B Virus in Hep一2 Cells
Virus Research Institute,Huhei Medica[University, Wuhan 430071
The dynamic change of coxsackie B virus(CBV)5 type propagation was studied in Hep一2 cells by immunofluorescence assay,reverse transcription polymerase chain reaction and cy—
tological methods The results showed that CBV RNA muld be detected at 4 hours in postinfec—
tion,vira1 antigen was positive at 8 hours and cytopathic effect could be found at 12 hours The
vira1 particles could be observed at 20 hours in postinfeedon These results provided new data for
observation of CBV propagation,also,it indicated that the reverse transcription polymerase chain
reaction was a sensitive technique for m onitoring CBV propagation
  Published online: 5 Jun 1999
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