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Virologica Sinica, 14 (2) : 123-128, 1999
Review
Preparation of Indirect ELISA Reagent to Diagnose TORCH Infection
tnggigute of Virology.HubeiAcademyof Medical Science,Wuhan 430079
 Correspondence:
(184.91KB)  
Abstract
Torch is the abbreviation of four pathgenic microbols: Tonoplasma gondic (Toxo), Rubella Virus (RuV), Cytomegalovirus (CMV), and Herpes Simplex Virus (HSV 1 2 ). A new indirect ELISA reagent to detect the specific IgG and IgM antibody to ToRCH has been prepared. McAbs, labeled with horseradish peroxidase, against human IgG and IgM are used as the second antibodies. Purified Toxo, RuV, CMV, HSV 1 2 are used as antigens to coat wells. The reagent's quality has been tested. The result showed that the reagent has high specificity and low background. The sensitivity is up to 1∶160~640, the precision is high and the coefficient of variation (C.V) is between 1.4%~9.0%. The reagent is stable (stored at 37 ℃ for 4 days, the change range of each target is no more than 15%).
  Published online: 5 Jun 1999
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