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Virologica Sinica, 14 (2) : 152-156, 1999
Rescue of Infectious Virions from Recom binant Plasmid with the Genome of Periplanta liginosa,Densovirus
1nsitute Virology,Wuhan University,Wuhan 430072
An infectious genome of the Periplanta fuliginosa densovirus (PfDNV) has been cloned into the bacterial plasmid pUC119. The viral genome could be rescued from the recombinant plasmid PfNDV pUC119 by transfection to sensitive Periplanta fuliginosa larvae. Virions can be found in the cells of the transfected larvaes by electron microscope, and the rescued virions were as infectious as wild type virions. In contrast, the viral genome could not be rescued from the recombinant plasmid deleted one extremity.
  Published online: 5 Jun 1999
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